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pmxpuromyocin backbone  (Addgene inc)


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    Structured Review

    Addgene inc pmxpuromyocin backbone
    Pmxpuromyocin Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmxpuromyocin+backbone/pMXs-Scl+(Plasmid+%23131601)/pm38755186-271-17-27
    Average 92 stars, based on 1 article reviews
    pmxpuromyocin backbone - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Retroviral:

    Article Title: Direct conversion of cardiac fibroblasts into endothelial-like cells using Sox17 and Erg.
    Article Snippet: .. Retroviral constructs and infection of fibroblasts Eight of the ten tested retrovirus constructs were cloned into the pMXpuromyocin backbone. pMX-Tal1 (#131601) and pMX-Klf2 (#50786) were purchased from Addgene. platE (Cell Biolabs #RV-101) cells were used to create the appropriate retroviruses for reprogramming with Nanofect (Alstembio #NF100) or Lipofectamine 2000 (Thermo Fisher #11668019) used to transfect the platE cells using the manufacturer recommended Nanofect or Lipofectamine protocols that has been previously described27. .. Retrovirus collected on Day 2 and Day 3 post-transfection are filtered using 0.45 micron PES syringe filtered, pooled, pelleted, and resuspended in neonatal fibroblast media with polybrene (EMD Millipore #TR-1003-G).

    Construct:

    Article Title: Direct conversion of cardiac fibroblasts into endothelial-like cells using Sox17 and Erg.
    Article Snippet: .. Retroviral constructs and infection of fibroblasts Eight of the ten tested retrovirus constructs were cloned into the pMXpuromyocin backbone. pMX-Tal1 (#131601) and pMX-Klf2 (#50786) were purchased from Addgene. platE (Cell Biolabs #RV-101) cells were used to create the appropriate retroviruses for reprogramming with Nanofect (Alstembio #NF100) or Lipofectamine 2000 (Thermo Fisher #11668019) used to transfect the platE cells using the manufacturer recommended Nanofect or Lipofectamine protocols that has been previously described27. .. Retrovirus collected on Day 2 and Day 3 post-transfection are filtered using 0.45 micron PES syringe filtered, pooled, pelleted, and resuspended in neonatal fibroblast media with polybrene (EMD Millipore #TR-1003-G).

    Infection:

    Article Title: Direct conversion of cardiac fibroblasts into endothelial-like cells using Sox17 and Erg.
    Article Snippet: .. Retroviral constructs and infection of fibroblasts Eight of the ten tested retrovirus constructs were cloned into the pMXpuromyocin backbone. pMX-Tal1 (#131601) and pMX-Klf2 (#50786) were purchased from Addgene. platE (Cell Biolabs #RV-101) cells were used to create the appropriate retroviruses for reprogramming with Nanofect (Alstembio #NF100) or Lipofectamine 2000 (Thermo Fisher #11668019) used to transfect the platE cells using the manufacturer recommended Nanofect or Lipofectamine protocols that has been previously described27. .. Retrovirus collected on Day 2 and Day 3 post-transfection are filtered using 0.45 micron PES syringe filtered, pooled, pelleted, and resuspended in neonatal fibroblast media with polybrene (EMD Millipore #TR-1003-G).

    Clone Assay:

    Article Title: Direct conversion of cardiac fibroblasts into endothelial-like cells using Sox17 and Erg.
    Article Snippet: .. Retroviral constructs and infection of fibroblasts Eight of the ten tested retrovirus constructs were cloned into the pMXpuromyocin backbone. pMX-Tal1 (#131601) and pMX-Klf2 (#50786) were purchased from Addgene. platE (Cell Biolabs #RV-101) cells were used to create the appropriate retroviruses for reprogramming with Nanofect (Alstembio #NF100) or Lipofectamine 2000 (Thermo Fisher #11668019) used to transfect the platE cells using the manufacturer recommended Nanofect or Lipofectamine protocols that has been previously described27. .. Retrovirus collected on Day 2 and Day 3 post-transfection are filtered using 0.45 micron PES syringe filtered, pooled, pelleted, and resuspended in neonatal fibroblast media with polybrene (EMD Millipore #TR-1003-G).



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    pmxpuromyocin backbone  (Addgene inc)
    92
    Addgene inc pmxpuromyocin backbone
    Pmxpuromyocin Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmxpuromyocin+backbone/pMXs-Scl+(Plasmid+%23131601)/pm38755186-271-17-27
    Average 92 stars, based on 1 article reviews
    pmxpuromyocin backbone - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier

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